The Effect Of Giving Sambiloto Leaf Extract (Andrographis Paniculata) On Collagenization And Histopathological Picture Of Skin Tissue In The Healing Process Of Dermapen Wounds In White Wistar Male Rats

Abstract

This study aims to analyze and test the administration of d extractAndrographis
paniculata (Andrographis paniculata) affect growth collagen in the wound healing
process of dermapen scars in male Wistar white rats (Rattus norvegicus) and assessing
its histopathological features. This study is a true experimental study, using a post-test
only control group design. The sample in this study was 24 animals. Preparation of test
animals is to increase the weight of mice and make dermapen wounds. Dermapen action
is carried out with a needle depth of 2 mm and a wound width of 2 cm. The wound healing
process is observed every day for 14 days. This study showed that the group given
Andrographis paniculata leaf extract cream with concentrations of 5%, 7%, and 10% had
an effect on the healing process of wounds from dermapen in obese white rats (Rattus
norvegicus) wistar strain. The treatment group experienced total healing, while the
control group given base cream did not. The results of histopathological observations
showed that the control group produced very thin collagen growth, while in the treatment
given Andrographis paniculata leaf extract with concentrations of 5%, 7%, and 10% the
collagen was fully filled and dense. The results of phytochemical tests showed that
Andrographis paniculata leaf extract contained secondary metabolites in the form of
flavonoids, saponins, tannins, and triterpenoids. These findings indicate that
Andrographis paniculata extract increases collagenization and accelerates wound
healing, making it a promising treatment for wounds caused by dermapen.