ANTIOXIDANT ACTIVITY AND EFFECTIVENESS TEST OF METHANOL EXTRACT SAMBILOTO LEAVES (Andrographis paniculata) ON WISTAR RATS (Rattus novergicus) EXPOSED TO ULTRA VIOLET RAYS

Abstract

Antioxidants can stop the damaging actions of free radicals. Antioxidants are divided
into two, namely enzymatic and non-enzymatic antioxidants.
This study aims to test the effectiveness of antioxidants and anti-aging of methanol
extract of sambiloto leaves (Andrographis paniculata) in preventing skin aging in
Wistar rats exposed to ultraviolet light. This study is an experimental study using pre
post test control group design. In this study, 24 male Wistar rats were used as samples
which were divided into 4 groups.
The results of the study showed that the active substances contained in the methanol
extract of sambiloto leaves (Andrographis paniculata) tested through phytochemical
testing contained secondary metabolites such as alkaloids, flavonoids, saponins, and
tannins. The results of the antioxidant test of the methanol extract of sambiloto leaves
(Andrographis paniculata) through the DPPH test obtained an IC50 value of 53.65. So
the antioxidant test of the sambiloto leaf extract (Andrographis Paniculata) showed that
there was an antioxidant content in the sambiloto leaf extract (Andrographis
paniculata) with a strong category. Seeing the antiaging activity of the methanol extract
of sambiloto leaves (Andrographis Paniculata) with the best collagen density area were
groups P3 and P2, namely the treatment groups given methanol extract of sambiloto
leaves (Andrographis paniculata) concentrations of 5% and 10% because the collagen
density was already very large and dense compared to treatment group 1 and the control
group in skin tissue histopathology. These results indicate that the methanol extract of
sambiloto leaves (Andrographis paniculata) has the potential as a natural antioxidant
and anti-aging agent for skin exposed to UV rays.