The Effect Of Giving Cloves Extract (Syzygium Aromaticum) On Collagenization And Histopathological Picture Of Skin Tissue In The Healing Process Of Dermapenese Wounds In White Wistar Male Rats

Abstract

Clove is a native plant of Indonesia but is currently cultivated in several parts of the world including Brazil in the state of Bahia. This plant represents one of the richest sources of phenolic compounds such as eugenol, eugenol acetate and gallic acid and has great potential for pharmaceutical, cosmetic, food and agricultural applications. The antioxidant and antimicrobial activities of cloves are higher than those of fruits. This study was conducted to test and analyze the effectiveness of giving clove extract cream (Syzygium aromaticum) on collagenization and wound healing from dermapen in male white rats (Rattus norvegicus) of the Wistar strain. The sample in this study was male white rats (Rattus norvegicus) of the Wistar strain weighing 160 – 200 grams and aged 2 – 3 months. Determination of the sample using the Ferderer formula for 4 groups and the overall results were 24 rats which would be divided into 4 different groups. Phytochemical test results showed that clove extract (Syzygium aromaticum) contains secondary metabolite compounds, namely flavonoids, saponins, tannins, alkaloids, and steroids which have antioxidant, antimicrobial, and anti-inflammatory effects and play a role in the healing process of dermapen wounds and collagenization. Clove extract cream (Syzygium aromaticum) with a concentration of 12% is most effective in accelerating wound healing, namely in 12 days. The results of observations of histopathological images of skin tissue showed that treatment groups 1, 2, and 3 with the administration of clove extract cream (Syzygium aromaticum) with concentrations of 4%, 8%, and 12% had a collagen density of 75 – 100 compared to the control group <25%.