The Effect Of Giving Sambiloto Leaf Extract (Andrographis Paniculatu) On Pancreatic Function And Pancreatic Histopathology In White Male Wistar Rats With Diabetes Mellitus

Abstract

Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose
levels caused by impaired insulin secretion from pancreatic β cells.
This study aims to evaluate the effect of Andrographis paniculata leaf extract on
pancreatic function and histopathological features of the pancreas of male Wistar white
rats with diabetes mellitus. This study is an experimental study using a pre-post test
control group design. The research sample of 24 Wistar rats was divided into 6 groups.
The results of observations conducted on all groups showed changes in amylase and
lipase levels in the treatment group. The results of the normality test of amylase and lipase
levels were said to be normally distributed because the p values were > 0.05, namely
0.100 and 0.436. Based on the differences in the average serum amylase levels, the
researchers concluded that the treatment group given Andrographis paniculata leaf
extract (Andrographis paniculata). dose of 600mg/KgBW had the highest decrease in
serum amylase and lipase levels. Histopathology of pancreatic tissue showed that the
treatment group given Andrographis paniculata leaf extract (Andrographis paniculata)
dose of 600mg/KgBW) category score 1 because the number of degenerative cells with
a degree of damage (<1-25%), the number of necrotic cells <25% and the number of
inflammatory cells <10 in the entire pancreatic interstitial space experienced the most
significant improvement. Sambiloto leaf extract (Andrographis paniculata) contains
secondary metabolites in the form of saponins, tannins, flavonoids, and triterpenoids
which help repair pancreatic cells damaged by diabetes mellitus.