The Effectiveness of Star Anise (Illicium Verum) Extract Nanoemulsion on Fibroblast Density and Collagen in the Skin of Male Wistar Rats (Rattus Norvegicus) Exposed to Ultraviolet Light

Abstract

UV radiation can also cause sun damage to the skin through DNA damage, inflammation, oxidative stress, which is a condition where the production of Reactive oxygen species (ROS) exceeds the body's antioxidant defenses, which causes cell damage.. This research aims toTesting and analyzing the effectiveness of nano emulsion of star anise (Illicium verum) extract on the number of fibroblast and collagen density in the skin of male white rats (Rattus norvegicus) Wistar strain after exposure to UVB rays.The sample of mice used was 25 in 5 groups (positive and negative controls, treatments 1, 2, and 3) where the extract preparation in the treatment was a nanoemulsion of star anise extract (Illicium verum) with a concentration of 10% and 20%. The results were Based on histological assessment, treatment group 2 also showed high collagen density with a score of 3, similar to the normal group and the positive control (vitamin C). In contrast, the negative control group experienced severe collagen damage with a score of 0. This indicates that a concentration of 20% is able to repair collagen tissue damaged by UVB exposure.