The Effect Of Hydrogen-Rich Water Administration On Serum Glutathione (Gsh) Levels And Lung Histopathology In Male Wistar Rats Exposed To Cigarette Smoke

Abstract

Environmental factors such as exposure to cigarette smoke can result in decreased antioxidant concentrations, one of which is: glutathione(GSH). Water with a higher pH and hydrogen ion content than regular water is known as hydrogen-rich water (HRW). Also one of the functions of hydrogen ions is as an antioxidant. This study was conducted to see how the effect of hydrogen-rich water on serum GSH levels and histopathological changes in the lungs of male Wistar rats exposed to cigarette smoke for 28 days. This study used 20 rats in 4 groups, control group 1.2 and treatment group 1.2. In this study, hydrogen-rich water (HRW) was given and serum GSH levels were examined using the ELISA method. Then observations were made after the experimental animals were exposed to cigarette smoke, which was then examined for lung histopathology in rats. The results showed a significant difference in serum GSH levels in the four groups of rats with a p-value <0.001 supported by a large effect size (ES = 0.935) so it can be concluded that HRW intervention provides a large antioxidant effect through serum GSH levels in rats and lung histopathology results also showed no significant difference in the degree of inflammation K1, K2, KP1, and KP2 with a p-value = 0.599 and a small effect size (ES = 0.418)