Synergistic Effects of Vitamin D3 and Vitamin E (α-tocopherol) on Haemoglobin Levels, Blood Glucose, and Neutrophil-Lymphocyte Ratio in Staphylococcus aureus Induced Septic Rats

Abstract

This study examines the combined effects of Vitamin D3 and Vitamin E supplementation on haemoglobin levels, blood glucose, and the neutrophil-to-lymphocyte ratio in septic rats caused by Staphylococcus aureus. The rats were categorised into seven groups: Group 1: Control group; Group 2: Sepsis group; Group 3: DOX group (9 mg/kg); Group 4: DOX (9 mg/kg) combined with Vitamin D3 (36 IU/kg) and Vitamin E (250 mg/kg); Group 5: Vitamin D3 (36 IU/kg) and Vitamin E (250 mg/kg); Group 6: Vitamin D3 (72 IU/kg) and Vitamin E (250 mg/kg); Group 7: Vitamin D3 (144 IU/kg) and Vitamin E (250 mg/kg) were administered orally for one week.  Blood samples were obtained to assess haemoglobin, glucose, and NLR levels. The research indicates that Staphylococcus aureus-induced sepsis markedly impacts haemoglobin levels and the neutrophil-to-lymphocyte ratio (NLR). Doxycycline, in conjunction with Vitamin D3 and Vitamin E, enhanced haemoglobin levels and decreased NLR. Nevertheless, no substantial variations in blood glucose levels were seen across the groups (p>0.05), although elevated doses of Vitamin D3 indicated some enhancement. The results indicate that the combination of Doxycycline with Vitamin D3 and Vitamin E may aid in the management of sepsis-related inflammation and the recovery of haemoglobin levels. Additional research is required to optimise dosage and therapy combinations.