The Effect Of Giving Clove (Syzygium Aromaticum) On Pancreatic Function And Pancreatic Histopathology Of Male Wistar White Rats Infected By Staphylococcus Aureus Bacteria

Abstract

Infection is still a health problem for developed and developing countries. The pancreas is one of the organs that often experiences infection. This study aims to analyze the effectiveness of giving clove extract (Syzygium aromaticum) on the pancreas function of male white rats (Rattus norvegicus) of the wistar strain infected with Staphylococcus aureus and how the histopathology is. The research sample was 24 male Wistar rats.

This study uses a quantitative experimental research type, namely by using a true experiment or laboratory experimental design. This study uses a post-test only control group design to determine and analyze the effects before and after administration of clove extract in improving pancreatic function in white rats (Rattus norvegicus) Wistar strain infected with Staphylococcus aureus bacteria. The results of the One-way Anova test on the results of observations of serum lipase and amylase levels showed a significance value of 0.000 or greater than 0.05. It was concluded that there was a significant difference between the control group, treatment group 1, treatment group 2, and treatment group 3. The Post Hoc LSD test showed that there was a significant difference between the control group and treatment group 1 (p = 0.000) and treatment group 2 (p = 0.000). There was no significant difference between the control group and treatment group 3 (p = 0.475). Clove extract contains secondary metabolites in the form of saponins, tannins, flavonoids, and triterpenoids that help repair damaged pancreatic cells due to Staphylococcus aureus infection. Administration of clove extract at a dose of 600 mg/kgBW is effective in improving pancreatic function in white rats (Rattus norvegicus) Wistar strain that experience Staphylococcus aureus infection. The results of histopathological observations of pancreatic tissue in treatment group 3 (600 mg/kgBW) experienced the most significant improvement and approached the control group (reference group) compared to other groups