Pengaruh Formulasi Cendawan Entomopatogen Metharizium anisopliae(Metchnikoff) Sorokin dan Beauveria bassiana(Bals.-Criv.) Vuill terhadap Pertumbuhan dan Daya Tahan Hama Penyakit pada Bibit Kelapa Sawit (Pre nursery)
DOI:
https://doi.org/10.34012/agroprimatech.v8i2.5409Keywords:
Palm Oil;, Metharizium anisopliae; , Beauvaria bassiana; , endophyte; , Growth Promotor;Abstract
Entomopathogenic fungi are fungi that can cause death to insect pests. Some reports state that entomopathogenic fungi are endophytic or can grow in plant tissue and are positive for plant growth and produce phenol substances that insects do not like. The study aims to determine whether the formulation of entomopathogenic fungi Metharizium anisopliae (Metchnikoff) Sorokin and Beauveria bassiana (Bals.-Criv.) Vuill given to pre-nursery oil palm seedlings can help increase growth and resistance to pests and plant diseases. The study used factorial RAK with 3 replications and 2 factors, namely M. anisopliae fungus (M) with 4 levels: M0 = 0 g (control), M1 = 5g, M2 = 10g, and M3 = 40g, and B. bassiana fungus (B) with 4 levels namely: B0 = 0g (control), B1 = 5g, B2 = 10g, and B3 = 40g. The research resultsshowed that sprouts could grow into seedlings well, with an average third leaves appearing at week 12 after planting. The results of ANOVA analysis showed that the treatment factor of M. anisopliae and B. bassiana fungus gave an effect that was not significantly different on the parameters of the number of leaves, plant height, stem diameter, leaf length, number of leaf spots, leaf spot area, number of insect bites, insect bite area, plant length, crown wet weight, crown dry weight, root wet weight, root dry weight and root crown ratio. In other words, adding various doses of mixed formulation of M. anisopliae and B. bassiana did not provide a response to trigger plant growth and prevent the undesirable effects of insects. There are suggested that the two fungi are already in the oil palm seedling tissue as endophytes but until the 12th week, they have not shown any effect as a growth promotor or preventive effect the seddling tissue not liked by insects.
References
Arab, Y.A., & El-Deeb, H.M. (2012). The use of endophyte Beauveria bassiana for bio-protection of date palm seedlings against red palm weevil and Rhizoctonia root-rot disease. Scientific Journal of King Faisal University (Basic and Applied Sciences) 13(2):91-101.
Araújo, W. L., Marcon, J., Maccheroni, W., Van Elsas, J. D., Van Vuurde, J. W. L., & Azevedo, J. L. (2002). Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Applied and Environmental Microbiology, 68(10): 4906–4914.
Azzahra N, Jamilatun M, Aminah A. 2020. Perbandingan pertumbuhan Aspergillus fumigatus pada media instan modifikasi carrot sucrose agar dan potato dextro agar. Jurnal Mikologi Indonesia 4(1):168-172. DOI: 10.46638/jmi.v4i1.69
Baehaki, S. E., Iswanto, E. H., & Munawar, D. (2016). Resistensi wereng cokelat terhadap insektisida yang beredar di sentra produksi padi. Jurnal Penelitian Pertanian Tanaman Pangan, 35(2), 106-107.
Behie SW, Jones SJ, Bidochka MJ. (2015). Plant tissue localization of the endophytic insect pathogenic fungi Metarhizium and Beauveria. Fungal Ecology 13:112-119. DOI: 10.1016/j.funeco.2014.08.001.
Behie SW, Mereita CC, Sementchoukova L, Barelli L, Zelisko PM, Bidochka MJ. (2017). Carbon translocation from a plant to an insect pathogenic endophytic fungus. Nature Cummunication 18:14245. DOI: 10.1038/ncomms/4245
Behie SW, Zelisko PM, Bidochka MJ. (2012). Endophytic insect parasitic fungi translocate nitrogen directly from insects to plants. Science 396:1576-1577. DOI:10.1126/science.1222289.
Branine, M., Bazzicalupo, A., & Branco, S. (2019). Biologi and aplications of endophytic insect-pathogenic fungi. PLOS Pathogens 15(9):e1007831. DOI: 10.1371/journal.ppat.1007831
Cai, N., Wang, F., Nong, X., Wang, G., Mc Neill, M., Cao, G., Hao, K., Shaofang, L., & Zhang, Z. (2019). Visualising confirmation of the endophytic relationship of Metarhizium anisopliae with maize roots using molecular tools and fluorescent labelling. Biocontrol Science and Technology. DOI: 10.1080/09583157.2019.1641792.
Darmawan, D. I., Setyorini, T., & Andayani, N. (2022). Respon pertumbuhan tiga varietas bibit kelapa sawit dengan pemberian pupuk bioslurry padat pada media tanam di pembibitan pre-nursery. Prosiding Seminar Nasional Instiper, 1(1), 85–93. DOI: 10.55180/pro.v1i1.235.
Dutta, P., Kaushik, H., Bhawmick, P., Puzari, K. C., & Hazarika, G. N. (2015). Metarhizium anisopliae as endophyte has the ability of plant growth enhancement. International Journal of Current Research, 7, 14300-14304.
Ferron, P. (1985). Fungal control. Comprehensive Insect Phisiology. Biochemical Pharmacology (12): 313−346.
Gana, L.P., Etsassala, N.G.E.R. & Nchu, F. (2022). Interactive effects of water deficiency and endophytic Beauveria bassiana on plant growth, nutrient uptake, secondary metabolite contents and antioxidant activity of Allium cepa L. Journal of Fungi 8:874. DOI: 10.3390/jof 8080874.
Giauque, H., & Hawkes, C. V. (2013). Climate affects symbiotic fungal endophyte diversity and performance. American Journal of Botany, 100(7): 1435–1444. DOI: 10.3732/ajb.1200568
Harefa, T. (2022). Penurunan Produksi dan Pendapatan Petani Akibat Intensitas Serangan Penyakit Ganoderma Boninense Pada Tanaman Kelapa Sawit (Studi Kasus di Aek Nabara Kecamatan Bilah Hulu Kabupaten Labuhan Batu Sumatera Utara) (Doctoral dissertation, Universitas Medan Area).
Ibrahim, E., Firmansyah., Mansur., & Prayogo, Y. (2022). Eksplorasi dan identifikasi morfologi cendawan entomopatogen isolat lokal Sulawesi Selatan sebagai calon biopestisida potensial. Buletin Palawija 20(2): 59-70.
Istifadah, N., Rohmah, N. D. N., & Suganda, T. (2022). Kemampuan air rendaman limbah media jamur tiram dan serbuk gergaji untuk mengendalikan penyakit bercak cokelat pada tanaman tomat. Jurnal Agrikultura, 33(2): 217-224. DOI: 10.24198/agrikultura.v33i2.40460
Korosi, G.A., Wilson, B.A.L., Powell, K.S., Ash, G.J., Reineke, A., Savocchia, S. (2019). Occurrence and diversity of entomopathogenic fungi (Beauveria spp. and Metarhizium spp.) in Australia vineyard soils. Journal of Invertebrate Pathology 164(2019):69-77. DOI: 10.1016/j.jip.2019.05.002.
Liu, Y., Yang, Y., & Wang, B. (2022). Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae play roles of maize (Zea mays) growth promoter. Scientific Reports, 12(1):15706. DOI: 10.1038/s41598-022-19899-7
Mahr, S. (2003). The Entomopathogen Beauveria bassiana. University of Winconsin, Madison. Diakses dari http://www.entomology.wisc.edu/mbc n/kyf410.html. (Diakses 14 Mei 2024).
Meyling, N.V., & Eilenberg, J. (2007). Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: potential for conservation biological control. Biological Control 43:145-155. DOI:10.1016/j.biocontrol.2007.07.007
Mesquita, E., Hu, S., Limu, T.B., Golo, P.S., & Bidochka, M.J. (2023). Utilization of Metarhizium as an insect biocontrol agent and a plant bioinoculant with special reference to Brazil. Frontiers in Fungal Biology 4:1276287. DOI:10.3389/ffunb.2023.1276287.
Novianti, D. (2017). Efektivitas Beberapa Media untuk Perbanyakan Jamur Metarhizium anisopliae. Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam, 14(2), 81-88.
Olaf, S. H. (2021). Eksplorasi dan sepeksi cendawan endofit pada tanaman cabai yang berpotensi sebagai entomopatogen (Doctoral dissertation, Universitas Andalas).
Pranata, F.S, Sidharta, B.B.R, Arsiningtys, I.S., Purwijatiningsih, E, Muwani, L.I., Retnaningati, D., Tobungan, N., & Widhiastuti, S.S. (2019). Petunjuk Praktikum Teknobiologi. Fakultas Teknobiologi, Universitas Atma Jaya. Gosyen Publishing: Yogyakarta. P:1-13.
Prayogo, Y. (2024). Cendawan Entomopatogen (CEP) Untuk Pengendalian Hama Penting Kedelai Dalam Mendukung Sistem Pertanian Berkelanjutan. Orasi Pengukuhan Profesor Riset Kepakaran Hama dan Penyakit Tanaman. Badan Riset dan Inovasi Nasional (BRIN): Jakarta. P 19-52.
Prayogo, Y. (2013). Patogenisitas Cendawan Entomopatogen Beauveria Bassiana (Deuteromycotina: Hyphomycetes) Pada Berbagai Stadia Kepik Hijau (Nezara Viridula L.) Jurnal Hama dan Penyakit Tumbuhan Tropika, vol. 13, no. 1, Mar. 2013, pp. 75-86, DOI:10.23960/j.hptt.11375-86.
Priwiratama, H., Rozziansha, T.A.P., & Pradana, M.G. (2023). Status terkini penyakit bercak daun kelapa sawit di Sumatera dan Kalimantan. Warta Pusat Penelitian Kelapa Sawit 28(1):27-38. DOI: 10.22302/iopri.war.warta.v28i1.101
Putri, S.A.R., Susanto, F.X.H., Tambun, S.H., & Oktiarso, T. (2022). Uji aktivitas antibakteri pada macam-macam media pada bakteri Escherichia coli dan Staphylococcus aureus dengan metode difusi agar dan dilusi cair. Jurnal Ilmiah Sain & Teknologi 2(2):1-15. DOI: 10.33479/sb.v2i2.153.
Santoso, T., Prijono, D., Wiyono, S., & Buchori, D. (2005). Development, uses and application of biopesticides in Indonesia: current research and future challenges biopesticides phytochemicals and natural products for the progress of mankind. Proceedings International Conference on Biopesticides, February 13-18, Chiang Mai, Thailand.
Sitinjak, E. S. (2018). Uji Efektifitas Jamur Entomopatogenik Metarhizium anisopliae dan Beauvaria bassiana Terhadap Mortalitas Larva Kumbang Tanduk (Oryctes rhinoceros) pada Chipping Batang Kelapa Sawit. Skripsi, Program Studi Agroteknologi, Fakultas Pertanian Universitas Medan Area Medan.
Valdes, R.C., Acosta, R.I.T., & Pena, S.R.S. (2022). Metarhizium acridum and other entomopathogenic fungi from grasshoppers at arid sites of northeastern Mexico. Southwestern Entomologist 47(3):547-558. DOI: 10.3958/059.047.0302
Vega, F.E., & Kaya, H. K. (2012). Insect Pathology. Ed ke-2. California: Academic Pr
Wardati, I., Erawati, D. N., & Salim, A. (2019). Perbanyakan Agens Hayati Cendawan Beauveria Bassiana Sebagai Pengendali Hama Penggerek Buah Kopi (PBKo) Di Desa Durjo Karangpring Kecamatan Sukorambi Kabupaten Jember. Seminar Nasional Hasil Pengabdian Masyarakat dan Penelitian pranata Laboratorium Pendidikan Politeknik Negeri Jember Tahun 2019, ISBN : 978-602-14917-8-2
Wilson, D. (1995). Endofit – evolusi istilah, dan klarifikasi fiksasi penggunaan dan definisinya. Oikos 73: 274−276. DOI: https://doi.org/10.2307/3545919
Yasmin, Y. & Fitri, L. (2010). The effect of Metarhizium anisopliae fungi on mortality of Aedes aegypti larvae. Jurnal Natural 10(1): 31-35.