Comparison of the effectiveness of robusta coffee bean extract and arabica coffee bean extract on the growth of Candida albicans
DOI:
https://doi.org/10.34012/bkkp.v4i1.6667Keywords:
antibacterial, arabica coffee beans, Candida albicans, inhibition, robusta coffee beansAbstract
Oral thrush, a fungal infection often associated with inadequate oral hygiene, necessitates treatment with antimicrobial agents. Extracts from Coffea canephora (Robusta) and Coffea arabica L. (Arabica) coffee beans contain bioactive compounds that demonstrate inhibitory activity against Candida albicans. This study investigated the comparative inhibitory efficacy of Robusta and Arabica coffee bean extracts on Candida albicans growth. A randomised, post-test only control group design was employed in this experimental laboratory study. Twelve treatment groups were established, and the disc diffusion method was used to assess antibacterial activity. Inhibition diameters were measured using a vernier caliper. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney tests. The mean inhibition diameters for Robusta coffee bean extract (Coffea canephora) were as follows: 9.28 ± 2.585 mm at 12.5% concentration, 12.35 ± 0.050 mm at 25%, 14.55 ± 0.050 mm at 50%, 16.50 ± 0.477 mm at 75%, and 18.55 ± 0.477 mm at 100%. For Arabica coffee bean extract (Coffea arabica L.), the mean inhibition diameters against Candida albicans were 8.10 ± 1.117 mm at 25% concentration, 10.91 ± 1.188 mm at 50%, 15.61 ± 2.115 mm at 75%, and 16.71 ± 1.980 mm at 100%. The positive control exhibited a mean inhibition diameter of 15.85 ± 0.180 mm. No inhibition was observed for Arabica coffee bean extract at 12.5% concentration or the negative control. The results indicate that Robusta coffee bean extract demonstrated a significantly greater inhibitory effect on Candida albicans growth compared to Arabica coffee bean extract. Liquid chromatography-mass spectrometry (LC-MS) analysis of both Robusta and Arabica coffee bean extracts revealed the presence of caffeine and chlorogenic acid, which likely contribute to their observed inhibitory activity.
References
Lalla VR, Patton LL, Dongari-Bagtzoglou A. Oral candidiasis: pathogenesis, clinical presentation, diagnosis and treatment strategies. Vol. 41, Journal of the California Dental Association. 2013. p. 263–8.
Toum ES, Cassia A, Bouchi N, Kassab I. Prevalence and Distribution of Oral Mucosal Lesions by Sex and Age Categories: A Retrospective Study of Patients Attending Lebanese School of Dentistry. Int J Dent. 2018 May 17;2018.
Verma S, Sharma H. Prevalence of Oral mucosal lesions and their association with Pattern of tobacco use among patients visiting a dental institution. Indian Journal of Dental Research. 2019 Sep 1;30(5):652–5.
Baty JJ, Stoner NS, Scoffield AJ. Oral Commensal Streptococci: Gatekeepers of the Oral Cavity. Vol. 204, Journal of Bacteriology. American Society for Microbiology; 2022.
Indrayati S, Sari IR. GAMBARAN Candida albicans PADA BAK PENAMPUNG AIR DI TOILET SDN 17 BATU BANYAK KABUPATEN SOLOK. Jurnal Kesehatan Perintis. 2018 Dec 28;5(2).
Makhfirah N, Fatimatuzzahra C, Mardina V, Hakim FR. PEMANFAATAN BAHAN ALAMI SEBAGAI UPAYA PENGHAMBAT Candida albicans PADA RONGGA MULUT [Internet]. Vol. 7, Jurnal Jeumpa. 2020 Dec. Available from: https://scholar.google.co.id/
Patel M. Oral Cavity and Candida albicans: Colonisation to the Development of Infection. Pathogens. 2022 Mar 10;11(3).
Hidayatullah T, Suci NL. ORAL THRUSH PADA BAYI: GAMBARAN KLINIS DAN TATALAKSANA (LAPORAN KASUS) ORAL THRUSH IN BABIES: CLINICAL APPEARANCE AND CASE MANAGEMENT (CASE REPORT). Cakradonya Dental Journal [Internet]. 2022;14(2):95–9. Available from: http://www.jurnal.unsyiah.ac.id/CDJ
Bintari Desi Wayan Ni, Setyapurwanti I, Devhy PLN, Widana OAA, Prihatiningsih D. SCREENING Candida albicans PENYEBAB KANDIDIASIS ORAL DAN EDUKASI ORAL HYGIENE PADA LANSIA DI PANTI SOSIAL TRESNA WERDHA WANA SERAYA BALI. Jurnal Pengabdian Kesehatan [Internet]. 2020 Jan 1;3(1). Available from: http://jpk.jurnal.stikescendekiautamakudus.ac.id
Sari ME, Fitriasari N, Nur’aeny N. Faktor risiko dan tatalaksana kandidiasis oral pada pasien dengan drug reaction with eosinophilia and systemic symptoms (DRESS). Jurnal Kedokteran Gigi Universitas Padjadjaran. 2022 Apr 28;34(1):80.
Suzuki BJ, Resnik RR. Wound Dehiscence: Incision Line Opening. In: Misch’s Avoiding Complications in Oral Implantology. Elsevier; 2018. p. 402–39.
Syakir M, Surmaini E. PERUBAHAN IKLIM DALAM KONTEKS SISTEM PRODUKSI DAN PENGEMBANGAN KOPI DI INDONESIA / Climate Change in the Contex of Production System and Coffee Development in Indonesia. Jurnal Penelitian dan Pengembangan Pertanian. 2017 Oct 27;36(2):77.
Wahyuni, Karim FS. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kacapiring (Gardenia jasminoides Ellis) terhadap Bakteri Streptococcus mutans. Jurnal Sains dan Kesehatan. 2020;2(4).
Rodiah AS, Fifendy M, Indriati G. Uji Daya Hambat Ekstrak Daun Beringin (Ficus Benjamina L.) Terhadap Pertumbuhan Jamur Candida albicans secara in Vitro. Serambi Biologi. 2022;7(4):318–25.
Pehino A, Fatimawali, Suoth JE. UJI AKTIVITAS ANTIBAKTERI EKSTRAK BIJI BUAH DUKU Lansium domesticumTERHADAP BAKTERI STAPHYLOCOCUS AUREUS DAN ESCHERICHIA COLI. Pharmacon. 2021 May 2;10(2).
Marbun TAR. Uji Aktivitas Ekstrak Daun Pirdot (Sauraia vulcani Korth.) Terhadap Pertumbuhan Candida albicans Secara In Vitro. J Bios Logos. 2020 Oct 15;11(1):1.
Meyok YG, Jasman. Uji Aktivitas Antijamur Minyak Kelapa Murni (Virgin Coconut Oil) Dan Minyak Kelapa Tradisional (Coconut Cooking Oil) pada Candida albicans. Jurnal Beta Kimia [Internet]. 2023 May 30;3(1). Available from: http://ejurnal.undana.ac.id/index.php/jbkHalaman|33
Martsiningsih AM, Suyana, Rahmawati U, Sujono, Astuti DF. PENGARUH WAKTU INKUBASI TERHADAP DIAMETER ZONA HAMBAT ANTIBIOTIK PADA UJI SENSITIVITAS BAKTERI KLEBSIELLA PNEUMONIA. The Journal of Medical Laboratory. 2023 Jun;11(1):2338–1159.
Ranasatri AA, Mahmudah N, Aisyah R, Sintowati R. AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70% BIJI KOPI ROBUSTA (Coffea canephora) TERHADAP Staphylococcus epidermidis DAN Salmonella typhi. Biomedika. 2021 Aug;13(2):101–10.
Daris SU, Syam H, Sukainah A. Uji Daya Hambat serta Penentuan Minimum Inhibitor Concentration (MIC) Dan Minimum Bactericidal Concentration (MBC) Ekstrak Daun Bidara Terhadap Bakteri Patogen. Jurnal Pendidikan Teknologi Pertanian [Internet]. 2023 Aug;9(2):223–34. Available from: https://journal.unm.ac.id/index.php/ptp/article/view/682
Rizki A, Handayani S, Widowati R. Daya Hambat Ekstrak Biji Kopi Robusta (Coffea canephora) Asal Bengkulu Terhadap Pertumbuhan Bakteri Penyebab Infeksi Kulit. Jurnal Penelitian Kesehatan Suara Forikes. 2022 Feb;13.
Rakatama SA, Pramono A, Yulianti R. The Antifungal Inhibitory Concentration Effectiveness Test From Ethanol Seed Arabica Coffee (Coffea arabica) Extract Against The Growth Of Candida albicans Patient Isolate With In Vitro Method. J Phys Conf Ser. 2018;970(1).
Sholichah E, Apriani R, Desnilasari D, Karim AM, Hervelly. BY-PRODUCT KULIT KOPI ARABIKA DAN ROBUSTA SEBAGAI SUMBER POLIFENOL UNTUK ANTIOKSIDAN DAN ANTIBAKTERI. Jurnal Industri Hasil Perkebunan. 2019 Dec;14(2):57.
Wigati IE, Pratiwi E, Nissa FT, Utami FN. UJI KARAKTERISTIK FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN BIJI KOPI ROBUSTA (Coffea canephora Pierre) DARI BOGOR, BANDUNG DAN GARUT DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl). FITOFARMAKA: Jurnal Ilmiah Farmasi. 2018 Jun 2;8(1):53–9.
Dewi ST, Chairani S, Rusdiana PRS. Perbandingan Jumlah Candida albicans Setelah Berkumur Ekstrak Jahe dan Klorheksidin pada Pengguna Ortodonti Cekat. Cakradonya Dental Journal [Internet]. 2023;15(2):81–6. Available from: http://www.jurnal.usk.ac.id/CDJ
Lubis AA, Cut SN, Mellaratna PW. Uji Daya Hambat Ekstrak Cabai Hijau (Capsicum annum L) Terhadap Pertumbuhan Jamur Candida albicans Secara In Vitro. Jurnal Penelitian dan Pengabdian Masyarakat. 2022 Nov;2(7).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Shirley Adriana, Pinta Malem Pinem, Desi Watri
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
